resDNASEQ NS0 and SP2/0 Residual DNA Quantitation kit

Features of the sDNASEQ  NS0&SP2/0 Residual DNA Quantitation kit include:

Simpler and Rapid

• Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
• Only one Reagent for qPCR;
• Only 1.5 hours will be needed for the whole test.

Accurate

• Perfect amplification curve, good amplification efficiency and good precision.
• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
•  Limit of Quantification (LOQ): 1fg/μL.
• The recovery rate of different concentration samples in the linear range is between 70% and 130%.

Kit Performance

Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.

Fig 2. All components of Sample Preparation Kit can be stored at room temperature.

Fig 3. Six concentration samples of 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 15%, Regression coefficient associated with standard solutions was 0.99995, and amplification efficiency was 100.430%.

Fig 4. Five concentration samples of 0.3fg/μL, 1fg/μL, 3fg/μL, 10fg/μL and 30fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentration values of samples with 1fg/μL and above concentrations were less than 15%.

Fig 5. Five samples of 0.03pg/μL, 0.3pg/μL, 3pg/μL, 30pg/μL and 300pg/μL were prepared, purified by sample preparation kit and detected by amplification kit. The recoveries of all simulated samples were between 80% and 120%.

Fig 6. Only one Reagent for qPCR MIX.