resDNASEQ Vero Residual DNA Quantitation kit

Features of the sDNASEQ  Vero Residual DNA Quantitation kit include:

Simpler and Rapid

• Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
• Only one Reagent for qPCR;
• Only 2 hours will be needed for the whole test.

Accurate

• Perfect amplification curve, good amplification efficiency and good precision.
• Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
• Limit of Quantification (LOQ): 0.3 pg/mL
• The recovery rate of different concentration samples in the linear range is between 70% and 130%

Kit Performance

Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.

Fig 2. Seven concentration Vero samples of 0.3fg/μL-300pg/μL were detected. Regression coefficient was closed to 0.9999. Amplification efficiency is 100.627%.

Fig 3. The CV of concentration values of samples with 0.3fg/μL and above concentrations were less than 30%. The accuracy deviation is within 30%.

Fig 4. Recoveries for seven simulated samples were consistently within the acceptable range of 70%–130%.

Fig 5. Recoveries for both simulated and actual samples were consistently within the acceptable range of 70%–130%.

Fig 6. The coefficients of variation (CVs) for within-run precision were less than 30%.

Fig 6. The coefficients of variation (CVs) for  total precision were less than 30%.

Fig 7. The one-component qPCR Mix reflects DuckyBio’s advanced technical capabilities.