Plasmid Residual DNA Quantitation kit

高精度质粒残留DNA定量试剂盒

快速取得成果和简化的工作流程

1. 告别复杂的样品制备和漫长的等待时间。我们的试剂盒只需3个简单的步骤即可完成样品制备（仅需20分钟即可完成）。

2.样品制备试剂盒的所有组件均可在室温下储存——无需昂贵的冷藏，简化了库存管理。

3.qPCR MIX 仅需一种试剂，整个测试（从样品制备到结果）仅需 1.5 小时——显著提高您的 QC 工作流程效率。

灵敏度和准确度

The kit provides a broad linear dynamic range of 1.5 copies/μL to 1.5 × 10⁶ copies/μL, covering the range of residual DNA concentrations typically encountered in bioproduction. With a standard-curve R² ≥ 0.999 and amplification efficiencies of 100.128% and 99.567%, the assay demonstrates excellent linearity, robust amplification performance, and high reproducibility, delivering consistent and reliable results across testing batches to support critical quality control decisions.

Leveraging proven TaqMan™ qPCR technology, this kit achieves a Limit of Quantification (LoQ) of 0.75 copies/μL of Linearized Plasmid and Limit of Quantification (LoQ) of 7.5 copies/μL of Circular Plasmid—enabling femtogram-level Plasmid DNA detection that outperforms industry standards.

Spiked sample tests confirm a recovery rate between 70% and 130%—ensuring accurate quantification even in complex matrices.

Why Choose Our Plasmid Residual DNA Kit?

Applications

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Frequently Asked Questions (FAQs)

1.What is the detection limit of the resDNASEQ Plasmid Residual DNA Quantitation Kit?

Answer: The kit offers an ultra-high sensitivity with  a Limit of Quantification (LoQ) of 0.75 copies/μL—making it capable of femtogram-level Plasmid DNA detection, which exceeds global regulatory requirements.

2.How long does it take to complete a full Plasmid residual DNA test with this kit?

答：整个过程仅需1.5小时。样品制备仅需20分钟（3个简单步骤），qPCR扩增和定量步骤无缝衔接——与传统试剂盒相比，节省了宝贵的时间。

3.该试剂盒是否适用于疫苗生产过程中残留DNA的检测？

答：是的，它广泛应用于疫苗生产的质量控制流程，包括病毒疫苗和亚单位疫苗。它能有效定量疫苗底物和最终产品中的质粒残留DNA，从而支持符合世界卫生组织和区域监管标准。

4.样品制备组件是否需要冷藏？

答：不——样品制备试剂盒的所有组件都可以在室温下储存，这简化了储存和运输，降低了冷链成本，并确保在标准实验室条件下的稳定性。

5.该试剂盒是否符合世界卫生组织关于残留质粒DNA的指导原则？

答：当然。该试剂盒是专门为满足世界卫生组织规定的灵敏度要求（每治疗剂量10纳克质粒DNA）而开发的，因此是生物制药和疫苗生产中符合监管要求的可靠选择。