Mycoplasma Detection Kit（qPCR）

Mycoplasma is the smallest and simplest prokaryotic organism. There is a risk of mycoplasma contamination during cell culture, and mycoplasma contamination of cells has become a common problem worldwide.
Mycoplasma contamination may seriously affect the state of cells, change the gene expression and metabolic characteristics of cells, lead to slow cell growth, abnormal differentiation and death, and seriously affect cell function.

1. Features of MycoSEQ Mycoplasma Detection Kit

• Broad Specificity: Detects over 90 species of Mycoplasma, Spiroplasma, and Acholeplasma with high specificity.
• High Sensitivity: Achieves a sensitivity with a limit of detection (LOD) down to 10 CFU/mL.
• Built-in Quality Control: An optional Positive Control (PC) is included.
• Inhibition Monitoring: Add the PC to the PCR reaction to identify false-negative results caused by reaction inhibitors.
• Extraction Efficiency Monitoring: Add the IPC prior to nucleic acid extraction to evaluate the efficiency of the extraction process.
• Simple & Rapid: Streamlined workflow delivers reliable results in under 2 hours post-extraction.

2.Contents and Storage

2. Performance

Fig 1. Six concentration Mycoplasma oralis samples of 1CFU/Reaction-10⁵ CFU/Reaction were detected. Regression coefficient was closed to 0.9993. Amplification efficiency is 99.802%.